Origanum majorana water extract protects against ethanol and indomethacin-induced gastric mucosal damage via decreasing oxidative stress and cytokine release

  • Mohey Ellithey Department of Pharmacology, National Research Centre, Cairo, Egypt
  • Sally Abdul Wanees El Awdan Department of Pharmacology, National Research Centre, Cairo, Egypt
  • Gehad Abdel Jaleel Department of Pharmacology, National Research Centre, Cairo, Egypt
  • Nermeen Shaffie Department of Pathology, National Research Centre, Cairo, Egypt
  • Omar ME Abdel-Salam Department of Toxicology and Narcotics, National Research Centre, Cairo
Keywords: Origanum majorana, gastric acid, cytokines, gastric mucosa, gastroprotection, indomethacin, ethanol, gastric ulcer

Abstract

The aim of this study was to investigate the effect of water extract of dried powdered leaves of Origanum majorana (O. majorana) on gastric acid secretion and experimental gastric ulcers in rats. Methods: O. majorana was given orally to rats by gastric tube in single doses of 50 mg or 100 mg/kg daily for seven days prior to pylorus ligation or ulcer induction. Gastric acid secretion was determined 4 h after pylorus ligation. Gastric mucosal lesions were produced by oral administration of  indomethacin (20 mg/kg) or ethanol (96%, 1 ml). Lipid peroxidation (malondialdehyde), reduced glutathione and the pro-inflammatory cytokines tumour necrosis factor-alpha (TNF-α) and inteuleukin-1beta (IL-1β) were determined in gastric mucosal homogenates. The effects of O. majorana extract were compared with that of the histamine H2-receptor antagonist ranitidine given orally at 50 mg/kg. Results: O. majorana given at 100 mg/kg to pylorus-ligated rats caused a significant decrease in gastric acid output by 33.5% and decreased the number and severity of gastric mucosal lesions caused by indomethacin or 96% ethanol.  O. majorana  decreased lipid peroxidation and the levels of the pro-inflammatory cytokines TNF-α and IL-1β in the gastric mucosa of rats given indomethacin or 96% ethanol. The extent of histological damage caused by indomethacin or 96% ethanol in the gastric mucosa was alleviated by O. majorana  in a dose-dependent manner with the higher dose of the extract restoring the normal structure of the mucosa. These effects of O. majorana water extract were comparable to that of the antiulcer drug ranitidine. It is concluded that O. majorana water extract exhibits gastric antisecretory and antiulcer effects. Inhibition of oxidative stress and decreased release of the pro-inflammatory cytokines TNF-α and IL-1β are likely to underlie the mucosal protective effects of Origanum majorana.
Published
2019-06-30
How to Cite
Ellithey, M., El Awdan, S. A. W., Abdel Jaleel, G., Shaffie, N., & Abdel-Salam, O. M. (2019). Origanum majorana water extract protects against ethanol and indomethacin-induced gastric mucosal damage via decreasing oxidative stress and cytokine release. Journal of Basic Pharmacology and Toxicology, 3(1), 19-28. Retrieved from http://scigreen.com/index.php/JBPT/article/view/55
Section
Original Research Articles