Separation and structural description of aloin and microdontin from leaf of Aloe yavellana reynolds

Abstract

Employment of succulent leaf of Aloe yavellana (Rey.), a ubiquitous plant species restricted to Sidamo floristic region, of Ethiopia, through preparative thin layer chromatography and using spectroscopic techniques led to the separation of two core tricyclic aromatic hydrocarbon (anthrones) compounds known as aloin (1), a light yellow powder; Rf = 0.351 (chloroform/methanol: 4:1); UV λmax (methanol): 357, 299, 208 nm; IR νcm‑1: 1085, 1291, 1618, 1631, 3400; ESIMS (-ve mode) with mass-to-charge ratio: 417 [M‑H]−, representing molecular formula C21H22O9 and molecular mass: 418 mu; and microdontin (2), a light yellow powder; Rf = 0.582 (chloroform/methanol 4:1); UV λmax (methanol): 311, 302, nm; IR νcm‑1: 1453, 1603, 1168, 1297, 1709, 3415; ESIMS (-ve mode)) with mass-to-charge ratio: 563 [M ‑ H]−, representing molecular formula C30H28O11 and  molecular mass: 564 mu.