Antibacterial and synergistic potency of methanolic leaf extract of Ocimum gratissimum L. and Vernonia amygdalina L.

  • Sylvester Chibueze Izah Department of Biological Sciences, Faculty of Science, Niger Delta University, Wilberforce Island, Bayelsa state, Nigeria
  • E.J. Uhunmwangho Department of Medical Laboratory Science, Faculty of Basic Medical Sciences, Ambrose Alli University, Ekpoma, Edo state, Nigeria
  • Nsikak Godwin Etim Department of Medical Microbiology, Federal Medical Centre, Yenagoa, Bayelsa State, Nigeria
Keywords: Medicinal plants, Vernonia amygdalina, Ocimum gratissimum, Bacteria pathogens, Antimicrobial activity

Abstract

Vernonia amygdalina and Ocimum gratissimum are common perennial shrubs that are used as vegetables and spices. Studies have indicated that both plants have several medicinal properties. This study investigated the antibacterial and synergistic potency of methanolic leaf extracts of Vernonia amygdalina and Ocimum gratissimum. The leaf samples were shade dried, extracted using methanol and tested against some bacterial isolates using agar well diffusion method. The mean zone of inhibition against E. coli, Staphylococcus aureus and Bacillus sp. was 13.67 mm, 13.33 mm and 13.00 mm, respectively by V. amygdalina, 13.00 mm, 12.67 mm and 13.00 mm, respectively by O. gratissimum, and 15.00 mm, 13.67 mm, 13.33 mm, respectively for synergy of V. amygdalina and O. gratissimum at 100% concentration. There was no significant variation (p > 0.05) among the various isolates, and plants and its combinations at 100% and 85% concentrations, but significant difference (p < 0.05) exists at 95% and 90% extracts. The findings showed that the combination had slight superior potency against the tested isolates.
Published
2018-01-20
How to Cite
Izah, S., Uhunmwangho, E., & Etim, N. (2018). Antibacterial and synergistic potency of methanolic leaf extract of Ocimum gratissimum L. and Vernonia amygdalina L. Journal of Basic Pharmacology and Toxicology, 2(1), 8-12. Retrieved from http://scigreen.com/index.php/JBPT/article/view/43
Section
Original Research Articles