Phenolic leaf extract of Vitex doniana ameliorated hepatorenal tissues dysfunctions in diabetic Wistar rats

  • E U Obasi Department of Biochemistry, Federal University of Technology, Owerri, Nigeria
  • K M E Iheanacho Department of Biochemistry, Federal University of Technology, Owerri, Nigeria
  • N Nwachukwu Department of Biochemistry, Federal University of Technology, Owerri, Nigeria
  • N C Agha Department of Biochemistry, Federal University of Technology, Owerri, Nigeria
  • Paul C Chikezie Department of Biochemistry, Imo State University, Owerri, Imo State
Keywords: Vitex doniana, hepatorenal, biomarkers, phenolic

Abstract

This study evaluated hepatorenal tissue biomarkers of diabetic rats treated with phenolic leaf extract of Vitex doniana. Diabetes mellitus (DM) was induced in the rats by a single dose of alloxan monohydrate (100 mg/kg i.p.). A total of 36 male Wistar rats were divided into 6 groups of 5 rats each. Separate DM-rat groups were treated with 100, 200 and 300 mg/kg i.p. of phenolic leaf extract of V. doniana as well as standard antidiabetic drug (metformin 500 mg/kg i.p.) every 12 hr for 28 days. Levels of serum hepatorenal tissues biomarkers, namely, alanine aminotransferase (ALT), aspartate aminotransferase (AST) and alkaline phosphatase (ALP) activities as well as serum concentrations of total bilirubin, total protein, albumin, urea and creatinine were measured using spectrophotometric methods. The results showed significant dose-dependent reductions (p < 0.05) in serum ALT, AST and ALP activities as well as total bilirubin, urea and creatinine concentrations in DM-rat groups treated with phenolic leaf extract of V. doniana. Furthermore, DM-rat groups treated with phenolic leaf extract of V. doniana, exhibited significant increases (p < 0.05) in serum total protein and albumin concentrations compared with untreated DM-rat group. The present study showed that phenolic leaf extract of V. doniana ameliorated hepatorenal tissues dysfunctions associated with alloxan-induced diabetes in rats.

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Published
2019-05-31
How to Cite
Obasi, E. U., Iheanacho, K. M. E., Nwachukwu, N., Agha, N. C., & Chikezie, P. C. (2019). Phenolic leaf extract of Vitex doniana ameliorated hepatorenal tissues dysfunctions in diabetic Wistar rats. Journal of Basic Pharmacology and Toxicology, 3(1), 7-13. Retrieved from https://scigreen.com/index.php/JBPT/article/view/54
Section
Original Research Articles